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<t>SDS-PAGE</t> gel with tricine buffer system and silver staining showing the presence of the lung surfactant proteins SP-B and SP-C in extracted porcine lung surfactant. The use of <t>the</t> <t>SDS-PAGE</t> clean-up kit to remove lipids, and the reductant DTT (dithiothreitol), before electrophoresis greatly improved the appearance of the bands, the process also removes the palmitoyl groups from the SP-C protein.
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<t>SDS-PAGE</t> gel with tricine buffer system and silver staining showing the presence of the lung surfactant proteins SP-B and SP-C in extracted porcine lung surfactant. The use of <t>the</t> <t>SDS-PAGE</t> clean-up kit to remove lipids, and the reductant DTT (dithiothreitol), before electrophoresis greatly improved the appearance of the bands, the process also removes the palmitoyl groups from the SP-C protein.
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SDS-PAGE gel with tricine buffer system and silver staining showing the presence of the lung surfactant proteins SP-B and SP-C in extracted porcine lung surfactant. The use of the SDS-PAGE clean-up kit to remove lipids, and the reductant DTT (dithiothreitol), before electrophoresis greatly improved the appearance of the bands, the process also removes the palmitoyl groups from the SP-C protein.

Journal: Environmental Science

Article Title: Changes to lung surfactant monolayers upon exposure to gas phase ozone observed using X-ray and neutron reflectivity

doi: 10.1039/d2ea00032f

Figure Lengend Snippet: SDS-PAGE gel with tricine buffer system and silver staining showing the presence of the lung surfactant proteins SP-B and SP-C in extracted porcine lung surfactant. The use of the SDS-PAGE clean-up kit to remove lipids, and the reductant DTT (dithiothreitol), before electrophoresis greatly improved the appearance of the bands, the process also removes the palmitoyl groups from the SP-C protein.

Article Snippet: Cleaning up the SDS-PAGE samples to remove associated lipids using an SDS-PAGE clean-up kit (GE Healthcare) greatly improved the band appearance.

Techniques: SDS Page, Silver Staining, Electrophoresis