Journal: Environmental Science
Article Title: Changes to lung surfactant monolayers upon exposure to gas phase ozone observed using X-ray and neutron reflectivity
doi: 10.1039/d2ea00032f
Figure Lengend Snippet: SDS-PAGE gel with tricine buffer system and silver staining showing the presence of the lung surfactant proteins SP-B and SP-C in extracted porcine lung surfactant. The use of the SDS-PAGE clean-up kit to remove lipids, and the reductant DTT (dithiothreitol), before electrophoresis greatly improved the appearance of the bands, the process also removes the palmitoyl groups from the SP-C protein.
Article Snippet: Cleaning up the SDS-PAGE samples to remove associated lipids using an SDS-PAGE clean-up kit (GE Healthcare) greatly improved the band appearance.
Techniques: SDS Page, Silver Staining, Electrophoresis